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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Gado de Leite. |
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Data corrente: |
17/03/2014 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAUJO, C. P.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; S. FILHO, A. F.; VIDAL, C. E. S.; ROXO, E.; NISHIBE, c.; ALMEIDA, N. F.; F. JUNIOR, A. A.; SILVA, M. R.; B. NETO, J. D.; CERQUEIRA, V. D.; ZUMÁRRAGA, M.; ARAUJO, F. R. |
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Afiliação: |
MARCIO ROBERTO SILVA, CNPGL. |
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Título: |
Detection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plos One, v. 9, n. 3, p. 1-6, 2014 |
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Idioma: |
Inglês
Português |
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Conteúdo: |
In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis. MenosIn the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with ... Mostrar Tudo |
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Palavras-Chave: |
Bovine and bubaline tuberculosis; Nested-PCR system. |
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Thesaurus Nal: |
Mycobacterium abscessus. |
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Categoria do assunto: |
--
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/119848/1/ARAUJO-C.-P..pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/99300/1/pone.0091023-1..6-2014-Nested-PCR.pdf
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Marc: |
LEADER 02739naa a2200325 a 4500
001 2010358
005 2024-02-05
008 2014 bl uuuu u00u1 u #d
100 1 $aARAUJO, C. P.
245 $aDetection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1.$h[electronic resource]
260 $c2014
520 $aIn the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis.
650 $aMycobacterium abscessus
653 $aBovine and bubaline tuberculosis
653 $aNested-PCR system
700 1 $aOSÓRIO, A. L. A. R.
700 1 $aJORGE, K. S. G.
700 1 $aRAMOS, C. A. N.
700 1 $aS. FILHO, A. F.
700 1 $aVIDAL, C. E. S.
700 1 $aROXO, E.
700 1 $aNISHIBE, c.
700 1 $aALMEIDA, N. F.
700 1 $aF. JUNIOR, A. A.
700 1 $aSILVA, M. R.
700 1 $aB. NETO, J. D.
700 1 $aCERQUEIRA, V. D.
700 1 $aZUMÁRRAGA, M.
700 1 $aARAUJO, F. R.
773 $tPlos One$gv. 9, n. 3, p. 1-6, 2014
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
05/12/2011 |
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Data da última atualização: |
09/02/2012 |
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Autoria: |
CANOSSA, R. S.; OLIVEIRA JUNIOR, R. S. de; CONSTANTIN, J.; RIOS, F. A.; CAVALIERI, S. D. |
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Afiliação: |
ROSECLER SALETE CANOSSA, UEM/NAPD; RUBEM SILVERIO DE OLIVEIRA JUNIOR, UEM/NAPD; JAMIL CONSTANTIN, UEM/NAPD; FABIANO APARECIDO RIOS, UEM/NAPD; SIDNEI DOUGLAS CAVALIERI, CNPH. |
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Título: |
Efetividade de herbicidas no controle de Alternanthera tenella. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Revista Brasileira de Herbicidas, Umuarama, v. 6, n. 1, p. 1-12, jan./jun. 2007 |
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ISSN: |
1517-9443 |
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Idioma: |
Português |
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Conteúdo: |
O objetivo deste trabalho foi avaliar a eficária de diferentes herbicidas, aplicados em pré e pós-emergência, no controle de Alternanthera tenella. Foram realizados dois ensaios em casa-de-vegetação: no primeiro, foram avaliados herbicidas aplicados em pré-emergência, e no segundo foram avaliados herbicidas em pós emergência, aplicados quando a planta daninha apresentava dois pares de folhas. |
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Palavras-Chave: |
Planta daninha. |
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Thesagro: |
Herbicida. |
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Categoria do assunto: |
W Química e Física |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/49259/1/Canossa-R.S.-Efetividade-de-herbicidas-no-controle-de-Alternanthera-tenella-2007.pdf
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Marc: |
LEADER 00989naa a2200205 a 4500
001 1908514
005 2012-02-09
008 2007 bl uuuu u00u1 u #d
022 $a1517-9443
100 1 $aCANOSSA, R. S.
245 $aEfetividade de herbicidas no controle de Alternanthera tenella.
260 $c2007
520 $aO objetivo deste trabalho foi avaliar a eficária de diferentes herbicidas, aplicados em pré e pós-emergência, no controle de Alternanthera tenella. Foram realizados dois ensaios em casa-de-vegetação: no primeiro, foram avaliados herbicidas aplicados em pré-emergência, e no segundo foram avaliados herbicidas em pós emergência, aplicados quando a planta daninha apresentava dois pares de folhas.
650 $aHerbicida
653 $aPlanta daninha
700 1 $aOLIVEIRA JUNIOR, R. S. de
700 1 $aCONSTANTIN, J.
700 1 $aRIOS, F. A.
700 1 $aCAVALIERI, S. D.
773 $tRevista Brasileira de Herbicidas, Umuarama$gv. 6, n. 1, p. 1-12, jan./jun. 2007
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Registro original: |
Embrapa Hortaliças (CNPH) |
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